Ejector refrigeration has the advantage of low capital cost, simple design, reliable operation, long lifespan and almost no maintenance. The only weakness is the low efficiency and its intolerance to deviations from design operation condition. R134a used in ejector refrigeration system gives better performance in comparison with many other environmental friendly refrigerants as the generation temperature is from 75°C to 80°C. The present work experimentally investigated the on-design and off-design performance of the ejector with fixed geometry using R134a as refrigerant, and cycle performance of the ejector refrigeration system. The experimental prototype was constructed and the effects of primary flow inlet pressure, secondary flow inlet pressure and ejector back pressure on ejector performance and cycle performance were investigated respectively. The operation conditions are: primary flow inlet pressure from 2.2 MPa to 3.25 MPa, secondary flow inlet pressure from 0.36 MPa to 0.51 MPa, ejector back pressure from 0.45 MPa to 0.67 MPa. Conclusions were drawn from the experimental results, and the experimental data can be used for validation of theoretical model for both critical and subcritical mode.